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Establishing Gd-IgA1 ELISA as a reliable and specific method to detect for Gd-IgA1. The horizontal axis represents concentration of Gd-IgA1 or intact IgA1 and the vertical axis represents OD. Enzymatically generated Gd-IgA1 (closed circles) was specifically recognized, whereas glycan-intact IgA1 from human plasma (open circles) was not recognized in the Gd-IgA1 ELISA (R = 0.99).
