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. 2015 Jun 3;66(16):5103–5112. doi: 10.1093/jxb/erv282

Fig. 1.

Fig. 1.

PIN2 protein levels are distinct in neighbouring epidermal cell files. (A) PIN2-GFP-expressing trichoblast and atrichoblast cell files display different levels of PIN2. (B) Atrichoblast cell files have a 20% stronger fluorescence signal at the plasma membrane than trichoblast cell files. A profile of fluorescence intensity (grey value) through the atrichoblast and trichoblast cell files is shown underneath. (C) Immunolocalization studies with a PIN2 antibody confirmed a stronger signal in atrichoblast cell files. A profile of fluorescence intensity through the cell files is shown underneath. (D) Quantification of fluorescence intensity at the plasma membrane revealed a 30% stronger signal at atrichoblast cell files. The data were statistically evaluated using Student’s t test. ***P <0.001; n=25 cells in five individual roots. Scale bar: 10 μm.