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. 2015 Jun 3;66(16):5103–5112. doi: 10.1093/jxb/erv282

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Interference with PIN2 trafficking abolishes differential PIN2 abundance in neighbouring epidermal cell files. (A) PIN2-VENUS as control at the plasma membrane. (B) Ubiquitinated form (PIN2-ubq-VENUS) of PIN2. (C) PM-stabilized form (pin2 ^12K-R-VENUS) of PIN2. (D) PIN2-VENUS shows a 30% stronger signal at the plasma membrane of atrichoblast cells. (E) PIN2-ubq-VENUS does not display differences at the plasma membrane in atrichoblast and trichoblast cells. (F) pin2 ^12K-R-VENUS also does not display differences in fluorescence intensity at the plasma membrane of atrichoblast and trichoblast cells. The data were statistically evaluated using Student’s t-test. ***P <0.001; n=25 cells in five individual roots. Scale bar: 10 μm.