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. Author manuscript; available in PMC: 2016 Jun 30.
Published in final edited form as: Biochemistry. 2015 Jun 19;54(25):4008–4018. doi: 10.1021/acs.biochem.5b00375

Figure 3. The metabolic intermediate acetyl-CoA acetylates mitochondrial aconitase and increases its activity.

Figure 3

Isolated and solubilized mitochondria were treated with 5 mM acetyl-CoA for 5min at room temperature. (A) Aconitase activity in mitochondria treated with acetyl-CoA compared to untreated controls. (B) Quantification of total acetylation status of aconitase measured by selected reaction monitoring of the acetylated peptides. The total acetylation measurement is determined as the geometric mean of the normalized abundance of all 21 acetylated peptides. Data are presented relative to the average of the untreated controls. (C) The abundance of the individual acetylated peptides measured by selected reaction monitoring. Data are presented relative to untreated controls. A log2 scale is used for the y-axis where a value of 1 represents no change from control. All values are presented as the mean ± SEM, where * indicates a significant increase in treated relative to untreated mitochondria (p < 0.05, n = 6).