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. Author manuscript; available in PMC: 2016 Jun 30.
Published in final edited form as: Biochemistry. 2015 Jun 19;54(25):4008–4018. doi: 10.1021/acs.biochem.5b00375

Figure 4. Mitochondrial aconitase activity increases in the cardiac tissue of mice fed a high fat diet for extended amounts of time.

Figure 4

Mice were fed a high fat or low fat control diet for up to 50 weeks, as indicated, and hearts were processed for functional analyses of mitochondrial aconitase. (A) Relative activity of aconitase in mitochondria from hearts of mice fed a high fat normalized to the control diet. (B) The corresponding mRNA levels by quantitative RT-PCR; and (C) the corresponding protein content determined by mass spectrometry. (D) Western blot analysis and densitometry for the expression of aconitase in high fat versus control diet for 2 weeks. All data are presented as the mean ± SEM, where * indicates a statistically significant increase in mice fed the high fat versus mice fed the control low fat diet at each time (p < 0.05, n = 5).