LC/ESI-MS/MS analysis of prostamides PGF2α-EA, PGE2-EA, and PGD2-EA in rabbit cornea. Sample reconstructed ion chromatograms of untreated corneal extract (baseline cornea) compared with corneal extract spiked with commercially available prostamide standards (spiked cornea) and standards (standard) at the following MRM transitions: PGF2α-EA (retention time 2.05 min): m/z 380 > 62 (A), m/z 380 > 283 (B), m/z 380 > 344 (C), and m/z 380 > 362 (D). PGE2-EA and PGD2-EA (retention times 2.15 min and 2.72 min, respectively): m/z 378 > 62 (E), m/z 378 > 299 (F), m/z 378 > 342 (G), and m/z 378 > 360 (H).