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. 2015 Aug;56(8):1419–1433. doi: 10.1194/jlr.M055772

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Copyright © 2015 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — Confirmation of rabbit corneal tissue capability to produce the prostamides PGF_2α-EA, PGE₂-EA, and PGD₂-EA. Sample reconstructed ion chromatograms of corneal homogenate incubated (10 min at 37°C) with externally added anandamide (10 μM and 50 μM; 10 A-EA and 50 A-EA) compared with commercially available prostamide standards (standard) at the following MRM transitions: PGF_2α-EA: m/z 380 > 62 (A), m/z 380 > 283 (B), m/z 380 > 344 (C), and m/z 380 > 362 (D). E: Product ions of m/z 380 corresponding to the corneal extract peak eluted at retention time (r.t.) 2.14 min (PGF_2α-EA). PGE₂-EA and PGD₂-EA: m/z 378 > 62 (F); m/z 378 > 299 (G); m/z 378 > 342 (H); and m/z 378 > 360 (I). J, K: Product ions of m/z 378 corresponding to the corneal extract peak eluted at retention times (r.t.) 2.24 min (PGE₂-EA) and 2.79 (PGD₂-EA).

Fig. 4. — Confirmation of rabbit corneal tissue capability to produce the prostamides PGF_2α-EA, PGE₂-EA, and PGD₂-EA. Sample reconstructed ion chromatograms of corneal homogenate incubated (10 min at 37°C) with externally added anandamide (10 μM and 50 μM; 10 A-EA and 50 A-EA) compared with commercially available prostamide standards (standard) at the following MRM transitions: PGF_2α-EA: m/z 380 > 62 (A), m/z 380 > 283 (B), m/z 380 > 344 (C), and m/z 380 > 362 (D). E: Product ions of m/z 380 corresponding to the corneal extract peak eluted at retention time (r.t.) 2.14 min (PGF_2α-EA). PGE₂-EA and PGD₂-EA: m/z 378 > 62 (F); m/z 378 > 299 (G); m/z 378 > 342 (H); and m/z 378 > 360 (I). J, K: Product ions of m/z 378 corresponding to the corneal extract peak eluted at retention times (r.t.) 2.24 min (PGE₂-EA) and 2.79 (PGD₂-EA).