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. 2015 Aug;56(8):1419–1433. doi: 10.1194/jlr.M055772

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Copyright © 2015 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 5. — The effect of COX inhibition on the formation of prostamides PGF_2α-EA, PGE₂-EA, and PGD₂-EA by rabbit corneal tissue. Sample reconstructed ion chromatograms of corneal extract incubated with exogenously added anandamide (50 μM, 10 min at 37°C) in the absence (cornea + A-EA) or presence of indomethacin (IND, 3 μM) (cornea + IND-A-EA) compared with commercially available prostamide standards (standard) at the following MRM transitions: PGF_2α-EA: m/z 380 > 62 (A) and m/z 380 > 283 (B); PGE₂-EA and PGD₂-EA: m/z 378 > 62 (C) and m/z 378 > 299 (D).