Fig. 2.
Disruption of intestinal Scap rapidly produces a severe enteropathy. A: Scapflox and Vil-Scap− mice (male, 10–12 weeks of age, 10 mice per group) were administered tamoxifen once daily for four doses as indicated by black arrows. Mice were euthanized and dissected on day 4 after the initial dose of tamoxifen and body weights measured. B, C: Gross appearance of viscera in situ and dissected gastrointestinal tracts from Scapflox and Vil-Scap− are shown. A green arrow indicates a fluid-filled small intestine. Yellow arrows indicate a dilated, food-filled stomach and shrunken, fluid-filled appendix. Statistical significance was assessed by the two-tailed Student’s t-test (A). * P < 0.05; ** P < 0.001.