Fig. 1.

Effect of ATV and LDL on Ldlr mRNA expression in fibroblasts. MEFs from WT and MLII mice were cultured in DMEM containing 10% NBCS or 10% LPDS supplemented with 10 µM ATV or 100 µg/ml human LDL for 48 h. The relative mRNA level of Ldlr and for comparison of Gnptab were determined by real-time PCR and normalized to β-actin mRNA expression. The relative mRNA expression in nontreated WT cells, incubated in DMEM containing 10% NBCS, was set at1. The data are the mean of triplicate PCRs obtained from three independent experiments and expressed as the fold change ± SD. * P ≤ 0.05, ** P ≤ 0.01, and *** P ≤ 0.005.