Table 2.

Table of human, mouse, zebrafish and frog microRNAs with seed sequences sharing at least 6 out of 7 nucleotides. The miRNAs significantly up-regulated in hESCsare highlighted in blue. Mature miRNA sequences are shown, with positions 1, 2–8, 9 and 10-end denoted. The chromosomal locations of the homologous human miRNA cluster are indicated at the left, and the arm of the precursor hairpin encoding the mature miRNA sequence is listed to the right of each sequence.

Species: human		Mature miRNA nucleotide number
	miRNA name	1	2–8	9	10-end	arm
chr 4	hsa-miR-302a	T	AAGTGCT	T	CCATGTTTTGGTGA	3prime;
	hsa-miR-302b	T	AAGTGCT	T	CCATGTTTTAGTAG	3’
	hsa-miR-302c	T	AAGTGCT	T	CCATGTTTCAGTGG	3′
	hsa-miR-302d	T	AAGTGCT	T	CCATGTTTGAGTGT	3′
chr7	hsa-miR-106b	T	AAAGTGC	T	GACAGTGCAGAT	5′
	hsa-miR-93	A	AAGTGCT	G	TTCGTGCAGGTAG	5′
chr 13	hsa-miR-17–5p	C	AAAGTGC	T	TACAGTGCAGGTAGT	5′
	hsa-miR-18a	T	AAGGTGC	A	TCTAGTGCAGATA	5′
	hsa-miR-20a	T	AAAGTGC	T	TATAGTGCAGGTAG	5′
chr 19	hsa-miR-372	A	AAGTGCT	G	CGACATTTGAGCGT	3′
	hsa-miR-373	G	AAGTGCT	T	CGATTTTGGGGTGT	3′
	hsa-miR-519a	A	AAGTGCA	T	CCTTTTAGAGTGTTAC	3′
	hsa-miR-519b	A	AAGTGCA	T	CCTTTTAGAGGTTT	3′
	hsa-miR-519c	A	AAGTGCA	T	Cl 111IAGAGGAT	3′
	hsa-miR-519d	C	AAAGTGC	C	TCCCTTTAGAGTGT	3′
	hsa-miR-519e	A	AAGTGCC	T	CCTTTTAGAGTGT	3′
	hsa-miR-520a	A	AAGTGCT	T	CCCTTTGGACTGT	3′
	hsa-miR-520b	A	AAGTGCT	T	CCTTTTAGAGGG	3′
	hsa-miR-520c	A	AAGTGCT	T	CCTTTTAGAGGGTT	3′
	hsa-miR-520d	A	AAGTGCT	T	CTCTTTGGTGGGTT	3′
	hsa-miR-520e	A	AAGTGCT	T	CC11111GAGGG	3′
	hsa-miR-526b*	A	AAGTGCT	T	CCTTTTAGAGGC	3′
Xchr	hsa-miR-106a	A	AAAGTGC	T	TACAGTGCAGGTAGC	5′
	hsa-miR-18b	T	AAAGTGC	A	TCTAGTGCAGTTA	5′
	hsa-miR-20b	C	AAAGTGC	T	CATAGTGCAGGTAG	5′
Species: mouse		Mature miRNA nucleotide number
	miRNA name	1	2–8	9	10-end	arm
chr 4	mmu-miR-302a	T	AAGTGCT	T	CCATGTTTTGGTGA	3′
	mmu-miR-302b	T	AAGTGCT	T	CCATGTTTTAGTAG	3′
	mmu-miR-302c	A	AGTGCTT	C	CATGTTTCAGTGG	3′
	mmu-miR-302d	T	AAGTGCT	T	CCATGTTTGAGTGT	3′
chr 7	mmu-miR-106b	T	AAAGTGC	T	GACAGTGCAGAT	5′
	mmu-miR-93	C	AAAGTGC	T	GTTCGTGCAGGTAG	5′
chr 13	mmu-miR-17	C	AAAGTGC	T	TACAGTGCAGGTAG	5′
	mmu-miR-18a	T	AAGGTGC	A	TCTAGTGCAGATAG	5′
	mmu-miR-20a	T	AAAGTGC	T	TATAGTGCAGGTAG	5′
chr 19	mmu-miR-290–3p	A	AAGTGCC	G	CCTAGTTTTAAGCCC	3′
	mmu-miR-291a-3p	A	AAGTGCT	T	CCACTTTGTGTGC	3′
	mmu-miR-291b-3p	A	AAGTGCA	T	CCATTTTGTTTGT	3′
	mmu-miR-292–3p	A	AAGTGCC	G	CCAGGTTTTGAGTGT	3′
	mmu-miR-294	A	AAGTGCT	T	CCCTTTTGTGTGT	3′
	mmu-miR-295	A	AAGTGCT	A	CTACTTTTGAGTCT	3′
Xchr	mmu-miR-106a	C	AAAGTGC	T	AACAGTGCAGGTAG	5′
	mmu-miR-18b	T	AAGGTGC	A	TCTAGTGCTGTTAG	5′
	mmu-miR-20b	C	AAAGTGC	T	CATAGTGCAGGTAG	5′
	mmu-miR-105	C	CAAGTGC	T	CAGATGCTTGTGGT	5′
Species-specific	mmu-miR-467a	T	AAGTGCC	T	GCATGTATATGCG	5′
	mmu-miR-467b	G	TAAGTGC	C	TGCATGTATATG	5′
	mmu-miR-467c	T	AAGTGCG	T	GCATGTATATGTG	5′
	mmu-miR-467d	G	AAGTGCG	C	GCATGTATATGCG	5′
Species: zebrafish		Mature miRNA nucleotide number
	miRNA name	1	2–8	9	10-end	arm
chr 7	dre-miR-93	A	AAAGTGC	T	GTTTGTGCAGGTA	5′
chr 13	dre-miR-17a	C	AAAGTGC	T	TACAGTGCAGGTA	5′
	dre-miR-18a	T	AAGGTGC	A	TCTAGTGCAGATA	5′
	dre-miR-20a	T	AAAGTGC	T	TATAGTGCAGGTAG	5′
Xchr	dre-miR-18b	T	AAGGTGC	A	TTTAGTGCAGATA	5′
	dre-miR-18c	T	AAGGTGC	A	TCTTGTGTAGTTA	5′
	dre-miR-20b	C	AAGGTGC	T	CACAGTGCAGGTAG	5′
Species-specific	dre-miR-430a	T	AAGTGCT	A	TTTGTTGGGGTAG	3′
	dre-miR-430ab	A	AAGTGCT	A	TCAAGTTGGGGTAG	3′
	dre-miR-430c	T	AAGTGCT	T	CTCTTTGGGGTAG	3′
	dre-miR-430i	T	AAGTGCT	A	TTTGTTGGCGTAG	3′
	dre-miR-430j	A	AAGTGCT	A	TCAAATTGGGGTA	3′
Species frog			Mature miRNA nucleotide nunber
	miRNA name	1	2–8	9	10-end	arm
chr7	xtr-miR-93a	A	AAGTGCT	G	TTCGTGCAGGTAG	5′
	xtr-miR-93b	A	AGTGCTG	T	TCGTGCAGGTAG	5′
chr 13	xtr-miR-17–5p	C	AAAGTGC	T	TACAGTGCAGGTAGT	5′
	xtr-miR-18a	T	AAGGTGC	A	TCTAGTGCAGATAG	5′
	xtr-miR-20a	T	AAAGTGC	T	TATAGTGCAGGTAG	5′
Xchr	xtr-miR-106	A	AAAGTGC	T	TATAGTGCAGGTAGA	5′
	xtr-miR-18b	T	AAGGTGC	A	TCTAGTGCAGTTAG	5′
	xtr-miR-20b	C	AAAGTGC	T	CATAGTGCAGGTAG	5′
Species-specific	xtr-miR-427	G	AAAGTGC	T	TTCTGTTTTGGGCG	3′