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. 2008 Feb 6;12(6a):2319–2333. doi: 10.1111/j.1582-4934.2008.00259.x

Figure 6.

Figure 6

Analysis of mouse MOR gene regulation by PARP-1 in vivo using siRNA. PARP-1 siRNA increases MOR transcription in NS20Y cells. (A) NS20Y cells were trans-fected with either scrambled siRNA (Scb) or PARP-1 siRNA (siPARP-1). Whole-cell extracts were made after incubation with the siRNAs for 48 hrs. Immunoblot analyses for PARP-1 and β-actin were performed. This figure is a representative of three separate experiments. (B) Quantification of MOR transcripts was performed by RT-PCR. Total RNA from NS20Y cells was prepared and treated with DNase I, and primer pairs specific for the coding sequence of each gene were used for RT-PCR. (C) Quantitative analysis using ImageQuant 5.2 software. The MOR mRNA levels from Control, scrambled (Scb) or siRNA-treated (siPARP-1) cells were normalized against β-actin levels. The values were obtained from triplicate data points and changes in transcript levels for Scb or siPARP-1-treated samples were compared to Control, which was assigned a value of 1.0. Bars indicate the range of standard error.