Figure 3.

PED expression correlates with TRAIL resistance in NSCLC cell lines. (A) Fifty micrograms of total cell extract from CALU-1, H460, A459 and A549 cells were analysed by Western blotting for the expression of PED and other TRAIL signalling molecules. β-Actin was used as the loading control. Representative blots are shown. (B) Viability after treatment with TRAIL. Cells were incubated with superkiller TRAIL (10 ng/ml) for 24 hrs and viability evaluated as described in the methods section. Mean ± SD of three independent experiments in triplicate. (C) Annexin V and propidium iodide staining of NSCLC cells after TRAIL treatment. Mean ± SD of four independent experiments in duplicate.