Figure 6.

Effects of PED on caspase activation. (A) CALU-1 cells were transfected with PED or control siRNA for 72 hrs and then treated with superkiller TRAIL for the indicated times. Lysates were examined by Western blotting with anti-caspase 8 or anti-PARP antibodies. Cleavage of caspase 8 and PARP was detected at a greater amount in CALU-1 cells transfected with PED siRNA. β-Actin was used as the loading control. (B) PED cDNA (PED-Myc) was transiently transfected in H460 cells and cells were analysed for caspase 8 and PARP activation as previously described or for or for cell viability (D) as indicated. (C) Western blot analysis of PED expression revealed that transfection increased PED expression levels in H460 cells. β-Actin was used as the loading control. Representative blots are shown.