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Changes of cytokines production. The bone marrow-derived cell (BMDC) supernatant incubated with 50 µg/mL mulberry leaf polysaccharide (MLP) for 48 h was assayed by ELISA for interleukin 12 (IL-12)p70 and tumor necrosis factor α (TNF-α)production, respectively. The absorbance number at 450 nm (A450) was then measured using a bichromatic microplate reader according to the instructions.
