Figure 2.

10% SDS-PAGE gel analysis of TBP reduced and ABD-F labeled monoclonal antibody h2E2 heavy and light chains. 0.2 mg/ml antibody was first reduced with 4 mM TBP at 60°C for the times indicated in the figure, followed by alkylation with 4 mM ABD-F for 15 minutes at 22°C. Aliquots (5 μg) of the resultant samples were diluted in SDS-PAGE sample buffer and run on a 10% gel. Following photography under UV light to detect incorporated ABD-cys fluorescence (left hand side); the gel was then stained to measure total protein, and re-photographed (right hand side). Migratory positions of the heavy and light chains are indicated.