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. 2015 Feb 18;11(2):458–467. doi: 10.4161/21645515.2014.990856

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Terence L Kirley and Andrew B Norman

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — 5% acrylamide non-equilibrium pH gel electrophoresis (NEPHGE) analysis of monoclonal antibody h2E2. NEPHGE was performed as described in Methods, with the gel run for 90 minutes at 200 V. Aliquots of 5, 10 and 20 μg of h2E2 antibody were analyzed, and the region of the gel containing the separated variants of the monoclonal antibody is expanded on the right side of the figure. Note the separation of several groups of charged variants of the antibody by this technique. BioRad IEF gel standards are shown in the left lane of the gel.