Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 18;11(2):458–467. doi: 10.4161/21645515.2014.990856

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Terence L Kirley and Andrew B Norman

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

PMC Copyright notice

Figure 4. — High performance strong cation exchange chromatography of the h2E2 antibody before and after treatment with carboxypeptidase B. 100 μg of h2E2 antibody was injected, and eluted with a gradient of NaCl in MES buffer. Note the disappearance of peaks 1 and 2 after removal of the C-terminal lysine residues. These peaks represent h2E2 antibody molecules containing 1 or 2 lysine residues on the C-termini of the 2 heavy chains in the antibody (which, after removal of lysine by carboxypeptidase elute with the main peak labeled ‘0” eluting at approximately 11 minutes).