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Reducing 10% SDS-PAGE analysis of h2E2 Fab, mAb and the Endo Lys-C digest. 5 μg of Fab, 6 μg of h2E2 mAb and 6 μg of Endo-Lys-C digested mAb were loaded onto lanes 2-4, respectively. Note the purity and lack of high molecular weight impurities in the Fab preparation. Migration positions of the reduced heavy and light chains, as well as the reduced Fab and Fc fragments, are indicated.
