Figure 6.

High performance strong cation exchange chromatography of the Fab fragment derived from the h2E2 antibody. 100 μg purified Fab fragment was injected, and eluted with a gradient of NaCl in MES buffer at 22°C. Note the relative lack of charge heterogeneity compared to the intact h2E2 antibody (Fig. 4 and the inset), indicating much of the charge heterogeneity resides in the Fc portion of the h2E2 antibody. For comparative purposes, the position and pattern of the intact antibody on the same column using the same buffers and gradient is shown in the inset (offset in the y-direction for clarity).