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Intrinsic h2E2 antibody tryptophan and tyrosine fluorescence quenching by cocaine binding. Shown are emission spectra of 5 nM h2E2 antibody both before and after addition of 100 nM cocaine, with excitation at either 280 nm (tyrosine and tryptophan) or 295 nm (tryptophan). Note the decrease in emission (quenching) caused by cocaine, but little if any change in the emission maximum (near 330 nm in both cases).
