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Selected PFGE patterns and MLST types from 7 leptospiral vaccine strains during in vivo and in vitro passages. The unweighted pair group method with arithmetic averages (UPGMA) was used as the clustering method for PFGE profiles. Number of passages: −1 represented vaccine strains before inoculation in animals; 1, 10 and 20 represented the first, tenth, and twentieth passage of each strain in culture, respectively.
