Figure 1.

IFN-γ production in primary mononuclear cell cultures challenged with Phytohaemaglutinin (PHA), Purified Protein Derivative (PPD) and M. bovis BCG. In the left panel, ELISPOT assay results for representative healthy adult mononuclear (A and B) and neonate cord blood cells (C and D) are shown. The corresponding production levels in terms of counts of spot forming colonies are described below. PHA, PPD and BCG antigens were used at concentrations of 1% and 5 μg/ml, respectively. In the right panel, the average productions (± SEM) for healthy adult mononuclear (HD) and neonate cord blood cells (UV) are shown.