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Analysis of crude T4 lysate collected during fermentation: (A) between 0 and 95 min; (B) between 120 and 205 min; (C) between 215 and 325 min. Conditions: Stationary phase: 0.34 mL CIM DEAE disk monolithic column; Mobile phase: Buffer A: 20 mM Tris, pH 7.5. Buffer B: 20 mM Tris, 1.0 M NaCl, pH 7.5; Gradient: linear gradient from 0 to 100% buffer B in 1.5 min; Flow rate: 4 mL/min; Injection volume: 1 mL; Detection: UV at 280 nm; reused from Smrekar et. al.44.
