The levels of serum IgG, IgG1, and IgG2a antibodies against CTT3H in different groups (n = 3). Three weeks after the last immunization, anti-CTT3H IgG, IgG1, and IgG2a (replaced by IgG2c) antibody titers in individual samples from different vaccinated C57BL/6 mice were determined by ELISA. The results were shown as mean (±SEM) log10 endpoint titers and the ratio of IgG2a/IgG1 of different vaccinated groups (n = 3). ─ means P < 0.05. This experiment was repeated twice with similar results.