Figure 7. Tumor growth after therapeutic E5, E6 and E7 immunization. Fifty micrograms of plasmid DNA were administered by electroporation on days 3 and 10 with either the indicated vaccine preparations or saline solution as control. (A) Cancer growth. Tumor development was assessed by palpation three times per week. (B) Tumor burden. Tumor volume was measured 4 wk p.c. and calculated as in “Material and Methods”. Bars (▬) represent the mean value per each mouse group.