Table 2.

Structure determination of recombinant virus-like particles or the subunit antigens of prophylactic vaccines against 3 viruses: HEV, HPV and HBV

		Methods of structural determination
Virus	VLP or capsid protein	X-ray	Cryo-EM	Reference
HEV	E2s (I)	√	×	14
	E2s (IV)	√	×	16
	p239	×	×	8
	T = 1 VLP (I)	√	√	91,92
	T = 1 VLP (III)	√	×	15
	T = 1 VLP (IV)	√	×	90
	T = 3 VLP (III)	×	√	92
HPV	Pentamer (HPV11,16,18,35)	√	×	96
	T = 1 VLP (HPV16)	√	×	95
	T = 7 VLP (HPV11,16)	×	√	19,67
HBV	HBsAg (Octahedral)	×	√	108