Table 2.

Linearity between the number of plasmid copies/reaction and Ct values to estimate the limits of detection and quantification for the Yellow Fever Virus RT-qPCR

Plasmid Copy Number (copies/reaction)	Ct mean	Standard Deviation	Number of positive replicates/Number of replicates
107	16.93	0.16	8/8
106	20.59	0.06	8/8
105	24.08	0.11	8/8
104	27.50	0.13	8/8
103	31.10	0.41	8/8
102	34.17	0.49	8/8 QL
50	34.88	18.07	5/8
25	35.53	18.40	5/8 DL
12.5	36.13	18.70	3/8
6.2	ND	-	0/8

Ct = Threshold Cycle; ND = Not Detected; QL = Quantification Limit; DL = Detection Limit.