Table 3.
Evaluation of the human RNase P gene as an endogenous internal amplification control in the TaqMan multiplex YFV RT-qPCR. Ct values for both RNAse P and NS5 region from yellow fever virus are indicated, as well as the standard deviation (SD). The samples were assayed in 8 replicates each
| Sample | Mean Ct (Yellow Fever Virus) | ± SD | Mean Ct (human RNase P) | ±SD |
|---|---|---|---|---|
| *Clinical sample (Low viral load) | 36.40 | 0.96 | 31.30 | 0.16 |
| *Clinical sample (Average viral load) | 35.84 | 0.83 | 31.41 | 0.02 |
| *Clinical sample (High viral load) | 34.95 | 0.83 | 31.93 | 0.02 |
| **Serological panel (Low viral load) | 35.94 | 0.23 | 30.22 | 0.11 |
| **Serological panel (High viral load) | 30.96 | 0.15 | 30.43 | 0.30 |
| Negative serum | ND | 30.86 |
Ct = Threshold Cycle; ND = Not Detected.
*
Pool of samples constituted by human sera from 17DD yellow fever virus vaccinated individuals.
**
Serological panel generated by spiking non-infected serum from a healthy individual (Negative Quality Control Serum) with purified 17DD yellow fever virus.