Figure 1. (A) Schematic of the expression cassette for Pfs25-FhCMB. The PR-1a signal sequence is followed by the lichenase gene fused to the N-terminal of Pfs25. A 6xHis affinity purification tag and the KDEL sequence are cloned to the C-terminus of Pfs25. (B) The deduced amino acid sequence for Pfs25-FhCMB. The PR-1a signal sequence, removed during in vivo processing, is shown in bold, the LicKM sequence is underlined, and the remaining portion is the Pfs25–6xHis-KDEL sequence.