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. 2015 Mar 9;11(4):1030–1035. doi: 10.1080/21645515.2015.1009809

Figure 1.

Figure 1.

IL-10R blockade enhanced CD8+ T cell responses to ChAdV63.HIVconsv in mice. (A) Gating strategy for the identification of IFN-γ+ CD8+ T cells. (B) IFN-γ and IL-2 production by HIVconsv-specific CD4+ T cells 21 d post-immunization. Mice were immunized with ChAdV63.HIVconsv in combination with anti-IL-10R (gray bars; n = 10) or isotype control antibody (white bars; n = 10). Statistical significance calculated using an unpaired t test (IFN-γ responses) or Mann-Whitney test (IL-2 responses). (C) Frequency of H-, P- and G1-specific IFN-γ+ CD8+ T cells 21 d post-immunization in mice immunized with ChAdV63.HIVconsv in combination with anti-IL-10R (gray bars; n = 10) or isotype control antibody (white bars; n = 10). (D) Total frequency of antigen-specific IFN-γ+ CD8+ T cells 21 d post-immunization in mice immunized with ChAdV63.HIVconsv in combination with anti-IL-10R (gray bar; n = 10) or isotype control antibody (white bar; n = 10). Data are representative of 2 independent experiments; statistical significance calculated using an unpaired t test. (E) Gating strategy for the identification of IFN-γ+ CD107a+ CD8+ T cells. (F) Total frequency of antigen-specific IFN-γ+ CD107a+ CD8+ T cells in mice immunized with ChAdV63.HIVconsv in combination with anti-IL-10R (gray bar; n = 10) or isotype control antibody (white bar; n = 10). Statistical significance calculated using an unpaired t test.