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. 2015 Mar 9;11(4):1030–1035. doi: 10.1080/21645515.2015.1009809

Figure 2.

Figure 2.

IL-10R blockade enhanced lysis of targets pulsed with a subdominant CTL epitope. (A) IFN-γ ELISPOT responses 21 d post immunization to 80 pools containing peptides spanning the HIVconsv immunogen in mice immunized with ChAdV63.HIVconsv in combination with anti-IL-10R (gray bars; n = 2) or isotype control antibody (white bars; n = 2). (B) IFN-γ ELISPOT responses to peptide 42 (left panel) and peptide 43 (right panel) in mice immunized with ChAdV63.HIVconsv in combination with anti-IL-10R (gray bars; n = 5) or isotype control antibody (white bars; n = 5). (C) Representative plot showing peptide 42-pulsed and unpulsed target cells isolated 16 hours after injection into a mouse immunized 21 d previously with ChAdV63.HIVconsv. (D) Proportion of peptide 42-pulsed targets killed in vivo in mice immunized 21 d previously with ChAdV63.HIVconsv in combination with anti-IL-10R (gray bar; n = 10) or isotype control antibody (white bar; n = 10). Statistical significance calculated using an unpaired t test.