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. 2015 Apr 6;11(6):1540–1556. doi: 10.1080/21645515.2015.1026500

Figure 8.

Figure 8.

Summary of functional epitope analyses. Results from 3 functional analyses are summarized according to frequency of HIV+ (lanes 1–4) or HIV− (lane 5) responders and are shown as (+) for frequency of >25 % responders, (±) for 19–25% responders, (−) for <19% responders, or as (*) for not available. Each lane, abridged in the insert, shows the ability of the peptide pool or peptide to stimulate an IFNγ response (lane 1), CD8+ T-cell proliferation (lane 2), and CD4+ T-cell proliferation (lane 3). Lane 4 denotes the ability to induce cytotoxin(s) in only CD8+ T cells (+) or in both CD8+ and CD4+ T cells (++) by 4 or more HIV+ subjects when at least 9 subjects tested. In lane 5, the positive result (+) indicates substantial frequency of proliferation responses from HIV− subjects and may indicate a safety concern; whereas a negative result (−) indicates no substantial HIV− response to the peptide pool or peptide. Positive response of HIV− control had CD8+ T-cell proliferation response in 30–42% of HIV− subjects. The large 13–15mer peptides with the best CMI responses without stimulation in HIV− subjects are shown with dashed boxes, while the best small peptides are shown with solid boxes. Therefore, Hp15–1, Hp15–2/3a, Fp14–4, Fp14–1b, Fp14–3/4e, and Fp14–3/4f peptides appear to contain the best potential epitopes to target for use as HIV immunogens.