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. 2015 May 27;11(5):1129–1139. doi: 10.1080/21645515.2015.1009343

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Figure 2. — Consistent characteristics of p239 VLPs in Samples a, c and c₀) undergoing different treatments. (A) HPSEC showed consistent antigen particle size (nearly identical retention time for Samples a, c and c₀). The molecular weight of p239 and E2 (dimer) were approximately 3,162 kDa and 147 kDa, respectively.⁷ Dimer, E2, in the assay serves as a control for elution pattern for a dimeric molecule, as opposed to a particulate antigen.²⁸ (B) AUC profiles revealed similar coefficients (21.8 s – 20.5 s) for p239 in 3 different samples. (C) Analogous pI (5.80 – 5.83) values of native and desorbed p239 VLPs were demonstrated by iCIEF.