Figure 5.

Immunochemical assays for p239 multi-faceted antigenicity analysis and comparable antigenicity by a sandwich ELISA. (A) mAb characterization and their applications in epitope mapping, SPR assay and sandwich ELISA for p239 antigenicity analysis. (B) The antigenicity of different p239 VLPs (Samples a, c and c₀) were measured using a monoclonal antibody-based sandwich ELISA (3A11 as the capture antibody, with 8C11 as the detection antibody). EC₅₀ values for different samples are reported in Table 1.