Figure 1.
Endothelial cells (ECs) in cultures. (A) Representative human microvascular ECs (HMECs) isolated from adipose tissue and used to prepare the SANTAVAC preparation. HMECs had numerous cytoplasmic extensions and/or a cobblestone-like morphology typical of adipose-derived microvascular ECs.89 Images were obtained using a Leica DM5000B microscope. Cells were isolated by using magnetic beads linked to anti-CD31 antibodies (visible on the cells). (B) Example of ECs sprouting around cancer cells under angiogenic stimuli. Artwork prepared using data obtained from a multiphoton microscopy image of a tumor spheroid in a collagen matrix.90