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Effect of melatonin pre-treatment on sperm morphological abnormalities in mice exposed to whole-body 60Co γ-irradiation. Animals were sacrificed through cervical dislocation and sperm was collected from cauda-epididymis on 1st, 3rd, 7th and 15th days post-irradiation. Single cell suspension of sperm was prepared in TNE buffer as well as prepared slide and stained with Eosin-Y. *p < 0.001, **p < 0.001
