Fig. 4.

Effect of rituximab on αCD20 scFv•apoA-I ND binding to B lymphoma cells in culture. Ramos and Granta cells were incubated in the presence or absence of rituximab for 45 min at 4 °C. After washing to remove unbound rituximab, the cells were incubated with αCD20 scFv•apoA-I ND. Cell-associated scFv•apoA-I was detected by FACS after incubation with a FITC labeled anti-apoA-I. Scans for each cell line are representative of 3 experiments. Incubation of αCD20 scFv•apoA-I ND without rituximab treatment is shown as broad black line. Histograms show the percent change in cell binding after rituximab treatment. Significance between rituximab treated and untreated cells is indicated.