Figure 1. Early appearance of foamy monocytes in blood of apoE−/− mice on western high-fat diet (WD).
A, Percentages of foamy monocytes in total monocytes in blood of apoE−/− mice at various time points (from 3 days to 10 weeks) after starting WD analyzed by flow cytometry. n=6–7 mice/group. B, Representative flow cytometric examples of monocytes in WT or apoE−/− mice on WD (for 3 days) from more than 5 independent experiments with 3–7 samples/group in each experiment. C, Percentages of CD11c+ monocytes in foamy, nonfoamy and total monocytes in blood of apoE−/− and WT mice on WD or normal diet (ND). n=6–7 mice/group. *P<0.05 versus WT WD and P<0.01 versus WT ND and apoE−/− ND; **P<0.01, ***P<0.001 versus WT ND, WT WD and apoE−/− ND; #P<0.05 versus WT ND and WT WD.