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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2015 Jun 25;35(8):1787–1797. doi: 10.1161/ATVBAHA.115.305609

Figure 5. Labeling of foamy monocytes and infiltration of foamy monocytes into nascent atherosclerotic lesions.

Figure 5

A, Representative flow cytometric analysis samples showing specific labeling of foamy monocytes and phenotypes of labeled foamy monocytes in apoE−/− mice on WD (3 weeks) after intravenous injection of DiI-CE-VLDLs with or without fluorescent microbeads, from more than 5 independent experiments with at least 3 samples in each experiment. B, Representative flow cytometric analysis samples showing foamy monocytes (DiI+F4/80low) in atherosclerotic aortas with sustained expression of CD11c, from 4 independent experiments with at least 3 samples in each experiment. C, Representative histology of aortic sinus showing accumulation of microbead+ (green) cells, which were also DiI+ (red, left panel) and CD11c+ (red, right panel), in atherosclerotic lesions of apoE−/− mice on WD (3 weeks) after intravenous injection of fluorescent microbeads with (left panel) or without (right panel) DiI-CE-VLDLs. D, Normalized frequency of infiltrated foamy monocytes in aortas of CD11c−/−/apoE−/− and CD11c+/+/apoE−/− mice on WD (3 weeks). n=5–7 samples/group. E, Representative histology and quantification of foamy monocyte infiltration in aortic sinus lesions of CD11c−/−/apoE−/− and CD11c+/+/apoE−/− mice on WD (3 weeks). n=15 samples/group.