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. Author manuscript; available in PMC: 2016 Jun 20.
Published in final edited form as: Am J Nephrol. 2015 Jun 20;41(0):392–399. doi: 10.1159/000433568

Figure 1. Early brodifacoum (BDF) effects on urinalysis and hematocrit.

Figure 1

The following number of animals was treated with BDF: 0.4 mg/kg n=16; 1.0 mg/kg n=6. Vehicle-treated rats (n=15) received the same volume of solvent.

A – a single dose of BDF was administered via oral gavage to rats in the doses shown. Urine was probed with a DiaScreen reagent strip (dipstick) at 30 min and hourly thereafter up to 4 hours after BDF administration. Results were graded using a semiquantitative scale of 0–3+. Score 0 was designated for no hematuria, score 1+ for mild hematuria, score 2+ for moderate hematuria and score 3+ for severe hematuria.

B – Hematocrit was determined in the blood samples at 30 min, 2 and 4 hours after BDF treatment.

C – free hemoglobin was measured in the serum using the Hemoglobin Assay Kit.

D – gross appearance of blood samples after 0.4 mg/kg (rat 1) and 1.0 mg/kg (rat 2) BDF treatment. Gross hemolysis was evident at 30 min and 2 hours after BDF administration.