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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Biol Psychiatry. 2015 Jan 24;78(6):396–402. doi: 10.1016/j.biopsych.2014.12.026

Figure 2. Redox dysregulation and loss of perineuronal nets (PNNs) enwrapping PV-cells.

Figure 2

(A) Double-labeling for 8-oxo-dG (green) and PV (red) in mature V1 (>P50) of WT (left) and PV-Gclc KO (right) mice. Higher magnification (inset) confirms elevation of oxidative stress in PV cells of PV-Gclc KO mice. (B) (left) Quantification (%) of PV+ cells also expressing high levels of 8-oxo-dG in PV-Gclc mice (69.3% KO vs 7.2% WT; P<0.000,1 t-test). (right) Total number of PV (+) cells, **p<0.01 t-test. (C) Double immunolabeling of PNNs with WFA (green) and PV (red) in mature V1 (>P50) of WT (left) and PV-Gclc KO (right) mice. Scale bar, 100 μm. (D) (left) Quantification of the number of WFA+ cells reveals loss of PNNs in PV-Gclc KO mice (vs WT; ***p<0.001, t-test). (right) Percentage of PV-cells enwrapped by WFA+ PNN also reduced in KO mice (**p<0.01, t-test).