Table 2. Primers used in PCR and sequencing.
| Target | Primer | Primer sequence* | Reference | |
|---|---|---|---|---|
| Tick | mt-rrs | mt-rrs(1) | CTGCTCAATGATTTTTTAAATTGCTGTGG | [8] |
| mt-rrs(2) | CCGGTCTGAACTCAGATCAAGTA | |||
| 12S rDNA | T1B | AAACTAGGATTAGATACCCT | [9] | |
| T2A | AATGAGAGCGACGGGCGATGT | |||
| Rickettsia | 17-kDa antigen | R1 | TCAATTCACAACTTGCCATT | [10] |
| R2 | TTTACAAAATTCTAAAAACC | |||
| gltA | RpCs877p | GGGGGCCTGCTCACGGCGG | [11] | |
| RpCs1258n | ATTGCAAAAAGTACAGTGAAC | |||
| ompA | Rr190.70p | ATGGCGAATATTTCTCCAAAA | [12] | |
| Rr190.602n | AGTGCAGCATTCGCTCCCCCT | |||
| Ehrlichia | groEL | gro607F a | GAAGATGCWGTWGGWTGTACKGC | [13] |
| gro1294R a | AGMGCTTCWCCTTCWACRTCYTC | |||
| gro677F b | ATTACTCAGAGTGCTTCTCARTG | |||
| gro1121R b | TGCATACCRTCAGTYTTTTCAAC | |||
| omp-1 | conP28-F1 a | ATYAGTGSAAARTAYRTRCCAA | [14] | |
| conP28-R1 a | TTARAARGYAAAYCTKCCTCC | |||
| conP28-F2 b | CAATGGRWGGYCCMAGARTAG | |||
| conP28-R2 b | TTCCYTGRTARGMAAKTTTAGG | |||
| Anaplasma | p44 | p3726 a | GCTAAGGAATTAGCTTATGA | [15] |
| p4257 a | AGAAGATCATAACAAGCATTG | |||
| p3761 b | CTGCTCKGCCAARACCTC | |||
| p4183 b | CAATAGTYTTAGCTAGTAACC |
*: M = A,C; W = A,T; K = G,T; R = A,G; S = C,G; Y = C,T.
a: 1st PCR.
b: nested PCR.