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. 2015 Jul 24;10(7):e0132644. doi: 10.1371/journal.pone.0132644

Fig 3. Morphometric analysis of vascular network remodeling after BRVO.

Fig 3

(A and B) Representative micrographs of CollIV-labeled retinal flatmounts of a control retina (A) and of the occluded vein after BRVO at 3d (B). Positions of the measurements at 800, 1200 and 1600μm from optic nerve were indicated by arrowhead. (C) Measurements of the retinal vein diameter of control retinas or of non occluded side and in the occluded vein at 1, 3 and 7d and indicated distance from the optic nerve after BRVO. Values in histograms are mean ± SEM of diameter (μm) of control or occluded vein in the retina from 4–11 eyes per group. Mann-Whitney non parametric test, * p<0.05 compared to control (0), non occluded veins of the same eye and intact eyes. (D and E) Representative micrographs of CollIV-labeled retinal flatmounts of the superficial capillary network of a control retina (D) and upstream of the occluded vein at 3d (E). (F and G) Quantification of the surface covered by CollIV-positive capillaries (F) and length (capillary length per surface) at different time points. Values in histograms are mean ± SEM of surface or length of capillaries in the retina from 4–18 eyes per group. Mann-Whitney non parametric test, * p<0.001 compared to control (0), non occluded veins of the same eye and non-occluded eyes. Scale bar = 100μm.