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. 2015 Jul 24;10(7):e0133205. doi: 10.1371/journal.pone.0133205

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© 2015 Hirano et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — (A) SA-β-gal staining at 48 h after knockdown of p21 and FLASH in MCs in the absence or presence of TNF-α shows induction of senescence. Representative data from one out of five independent experiments are shown. (B) The number of cells was determined microscopically using a hemocytometer at the indicated conditions. (C) Cells were counted on the basis of their morphological changes and expressed as the percentage of the total cell population. Results represent as arbitrary units and are shown as mean values ± SDs of at least three independent experiments. *P<0.001.