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NF density is decreased at the proximal level (50μm) of optic axons in HKO mice compared with those in WT mice (A, B). The lower panel of A is the higher magnification of the marked areas in the upper panel. Scale bar, 100 nm. Decreased NFL in the most proximal segment of optic nerve (500μm) confirmed in immunoblot analyses of consecutive 500μm segments using an anti-NFL antibody along optic axons (C).
