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. 2015 Jul 24;10(7):e0132434. doi: 10.1371/journal.pone.0132434

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© 2015 Schyth et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Microarray analysis of miRNA regulation in liver samples from diseased rainbow trout infected with Viral hemorrhagic septicaemia virus (VHSV). The Ncode V2 probe set covering miRBase v.9 was used to detect up-(blue) and down-regulated (red) miRNAs. The miRNA probes are denoted according to the miRBase nomenclature. Organisms: dre = Danio rerio, hsa = Homo sapiens and dme = Drosophila melanogaster. (B) qPCR validation of miR-462 and miR-731 expression in the liver of VHSV-infected rainbow trout. Fold regulation was calculated from the mean values of duplicate measurements using the ΔΔC_t method, using the omy-snoRNA U23 for normalization. miRNA expression was analyzed in 6 VHSV-infected fish relative to 6 uninfected fish.