. 2015 Jul 24;10(7):e0133855. doi: 10.1371/journal.pone.0133855

Table 2. Oligonucleotide primers for cloning.

Name	Sequence (5’-3’)	Purpose
HmbR-Nterm	CACCATGAAACCATTACAAATGCTCCC	Cloning of 5’ end of hmbR
dhmbR-rev	GCTGGATCCC AATCTGTATC	Cloning of 5’ end of hmbR
HmbR-Cterm	TTAAAACTTCCATTCCAGCG	Cloning of 3’ end of hmbR
dhmbR-for	TGGGATCCATTCACGGTTACGC	Cloning of 3’ end of hmbR
HpuA-Nterm	CACCGCCGAACCGCACGTCCCCG	Cloning of hpuAB
HpuB-Cterm	TTAGAACTTCGCTTCGATGG	Cloning of hpuAB
HA-Inv938	CTGATATCAGTGTCCCGGTAGCCG	Deletion of internal hpuAB fragment
HB-Inv1	CGGATATCGACTGGCGGTTTACCAAG	Deletion of internal hpuAB fragment
HpuA-N-Pleic	TACTTCCAATCCATGGCCGAACCGCACGTCCCCGTGT	Cloning of HpuA into expression vector
HpuA-C-Pleic	TATCCACCTTTACTGTCAGGGAAACGCTTGGGCGAT	Cloning of HpuA into expression vector
HpuB-N-Pleic	TACTTCCAATCCATGGCGTTTCCCGCCTTTGCGGCAGA	Cloning of HpuB into expression vector
HpuB-C-Pleic	TATCCACCTTTACTGTCATTAGAACTTCGCTTCGATGGT	Cloning of HpuB into expression vector
HmbR-N-Pleic	TACTTCCAATCCATGGCAGATGAAGCTGCAACTGA	Cloning of HmbR into expression vector
HmbR-C-Pleic	TATCCACCTTTACTGTCATTAAAACTTCCATTCCAGCGAT	Cloning of HmbR into expression vector
HmbR loop2 F	GAAGGGGAAGGCAGTGGCGCGAATATCCGTGGTTCGGCACGCGGTATCCCTGATTCGTCCAAACACTTAGGCGCTGGTGATTTGCAA	Cloning of loop 2 of HmbR
HmbR loop2 R	GCCACTGCCTTCCCCTTCCACAGCATAGCCTCGGTTTCCCGCACTTTCGGTTTCATGACCGCGAGCAATTTTAGCATCTACAAG	Cloning of loop 2 of HmbR
HmbR loop8 F	ACTGAGGAAAATGCTTACTACGGTATATGCAGCGACCCCTACAAAGAAAAATTAGGCGCTGGTGATTTGCAA	Cloning of loop 8 of HmbR
HmbR loop8 R	GTAAGCATTTTCCTCAGTACAGCCGGGAGTGCCGCTGGTGGTCAGCTTCTGAGCAATTTTAGCATCTACAAG	Cloning of loop 8 of HmbR
HmbR loop11 F	GATGGCAAAGGCTTAGACCGCTACCGCGCCCCAGGCCGCAATTTAGGCGCTGGTGATTTGCAA	Cloning of loop 11 of HmbR
HmbR loop11 R	GTCTAAGCCTTTGCCATCGCGGTCGACCGCATTGGTGGTGCTAGCAATTTTAGCATCTACAAG	Cloning of loop 11 of HmbR