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. 2015 Jul 24;10(7):e0133040. doi: 10.1371/journal.pone.0133040

Fig 4. Nasal slice cultures exposed to 1,8-cineol show reduced activity of NF-κB.

Fig 4

A Immunocytochemistry of LPS-treated nasal slice cultures revealed nucleus localization of NF-κB-p65 (upper panels, arrows). Co-treatment with LPS and 1,8-cineol resulted in reduced amounts of nuclear NF-κB-p65 (lower panels, arrows) and localization of NF-κB-p65 in the cytoplasm (lower panels, arrowheads). Scale bar: 20μm. B: Quantification of immunocytochemical analysis showed significantly increased numbers of epithelial cells with cytoplasmic NF-κB-p65 after LPS and 1,8-cineol co-treatment in comparison to the LPS-approach, indicating a significantly reduced NF-κB-activity. ***p < 0.001 was considered significant (t-test). C: Schematic view of NF-κB activating MUC2 gene expression via binding to a κB-binding site in 5’ region of the MUC2 gene (31).