(A) Diagrams showing the parents and process for the construction of the three F1 hybrids, designated as Hybrid 1, Hybrid 2, and Hybrid 3, respectively. (B) Typical chromosomal constitutions of each of the three F1 hybrids, as revealed by multicolor GISH. The pink-, green-, and blue colored chromosomes represent the AA, CC, and the other genomes, respectively. The sphere-shaped clump is another intact nucleus in which though the chromosomes were not separated, their three coloration can also been seen. The photos from top to bottom are Hybrid1, Hybrid 2 and Hybrid 3, respectively. (C) Phenotypic characteristics of F1 hybrids and their parents; a, d, g are Hybrid 1, b, e, h are Hybrid 2, c, f, i are Hybrid 3. From left to right in Fig 1C were maternal Nipponbare, paternal wild rice and the corresponding F1 hybrids.